could you please check if, by coincidence, one of your hard drives, flash drives, etc. is named AmiKit ? If so, rename it please.
Also check where you installed AmiKit. Does it have full permissions there? Could you re-install it to, eg. D: drive?
Also it might help to update to the latest WinUAE. Please follow the Quick Guide instructions on page 12: http://bit.ly/AmiKit-QuickGuide
If none of the above help, please send me email to firstname.lastname@example.org and I could check your AmiKit installation remotely via TeamViewer if you agree https://www.teamviewer.com/