you can drag and drop any folder onto AppAssign icon to create an Assign to that folder. For example, if you create a folder named RAM:ThisIsMyFolder and then drag&drop it onto AppAssign, it lets you create an assign named eg. "Mine". From now on, anytime you call "Mine", eg. in Shell, it points you to RAM:ThisisMyFolder location.
Anyway, move AppAssign from AmiKit:WBStartup folder to AmiKit:WBStartup/Disabled folder to prevent AppAssign from running on system startup.