This is maybe a dumb question but have you installed your AF2005Online before running AmiKit?
Have you also installed AmiKit 1.02 update before running AmiKit? It fixes the problem with AF2005 recognition. Make sure the update is installed to correct path, eg. if Amikit was installed to
then point the v1.02 update to the same directory.
Anyway, if it doesn't help, once you have installed AF2005online, run the AmiKit launcher only (the Win GUI with those 5 buttons). Do NOT press "Run AmiKit". Once the GUI is started and AF2005 is found on your system, a file containing a path to the system files of AF2005 is created inside the AmiKit directory. Check this file (AmiKit\Prefs\Env-Archive\AFOnlinePath) whether it contains the correct path. If not, fix it and press "Run AmiKit" button... I hope it helps.